• Imaris Scene Viewer


• Load Imaris Scenes (all Formats)
• Read Only (no option to modify and change image or scene data)
• Snapshot
• Animation Playback
• Rotate, Pan, Zoom


• Single File for Image Data and Surpass Scene

• Extended Imaris 5.5 File Format
• Fast "Save" (saves only if modified: image, metadata, or scene)
• Compatible with old Imaris Versions (Imaris 5.5 ignores the scene data)
• Option to export or import "old" IMX-Files


• RGB Segmentation

• Creates a new channel using a reference color and any number of input channels. The intensities of the new channel reflect the distance from the reference color. A high intensity in the new channel means that the color is very similar to the reference color.
• Multithreaded (processing is performed on multiple CPUs)


• Histogram Equalization
• See Wikipedia
• Multithreaded (processing is performed on multiple CPUs)


• Accelerated "Normalize" (multithreaded)
• Automated Crash Reports
• Updated Manual with ALL the new features

• Compact GUI for Lower and Upper Threshold

• Recenter (moves manually set spots to the correct position)
• Track Single Spot (very fast for large images, if only some spots are of interest)
• New Statistics Values (see MeasurementPro)

• Unify, Split (connects spacial separated objects)
• Contours integrated into Edit-Tab (see MeasurementPro)
• New Statistics Values (see MeasurementPro)

Image above: A Neuron traced with the new Filament Creation Wizard. The Spines are colored using the Branch Level of the Dendrites. Red Spines are attached to low level branches (primary), and yellow Spines are attached to high level branches. Image courtesy of Kim Edward Light, Professor Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciencies
• Separated Dendrites and Spines Detection:
• Dendrites: Source Channel, Diameter for Beginning- and Seed-Points
• Spines: Source Channel, Diameter for Seed-Points
• Clean Unwanted Seed-Points near Beginning-Point
• Interavtive Diameter Preview
• Option to Calculate Diameter from Cross-Section (increases accuracy of volume measurements)
• Accelerated Processing Speed for "Fast Marching Algorithm"
• New Statistics Values (see MeasurementPro)

• New Draw Modes: Circle, IsoLine, MagicWand
• Autofit (Fitting manual contrours to image gradients using snakes)
• Automatic / Manual Resolution for Surface Generation
• Surface Generation requires less memory
• Translate & Rotate Contour Lines
• Many keyboard shortcuts for more efficient use
• COM Interface (see ImarisXT)

• Colored Tracks by Statistical Value (e.g. Speed):



• Category Pre-Filter (Filter-Tab, Statistics-Tab):



• Statistics Tab:


• Icon for indicating a Statistics Value with Time Plot
• New Time Plots:
• Detailed / Average
• Selection / Specific Value
• Selection / Average Values
• Selection / All Values
• Export Plot Data
• Statistics Tab on "Surpass Scene" Object (Group)


• New Spots Statistics Values:
• Acceleration
• Track Position X Mean
• Track Position Y Mean
• Track Position Z Mean
• Track Speed Min
• Track Speed Max
• Track Speed StdDev


• New Surfaces Statistics Values:
• Acceleration
• Number of Disconnected Components
• Number of Disconnected Components per Time Point
• Total Number of Disconnected Components
• Track Position X Mean
• Track Position Y Mean
• Track Position Z Mean
• Track Speed Min
• Track Speed Max
• Track Speed StdDev


• New Filament Statistics Values:
• Spine Neck Volume
• Spine Terminal Pt. Volume

• Interface for Contour Polygon Coordinates
• XTension to Track Spine Heads, Dendrite Terminal- and Branch-Points
• Compiled Pre-XTensions (requires installation of Matlab Runtime)

Any feedback on the installation, and the usage of Imaris and its features are welcome. Please send your comments to eusupport@bitplane.com